Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes
نویسندگان
چکیده
منابع مشابه
Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody.
A monoclonal antibody (MAb) immunoglobulin G2a (2125) was produced against a 60-kDa Legionella heat shock protein (HSP), recognizing a unique epitope common to all species of the genus Legionella. The antibody reacted in the immunoblot with 59 Legionella species and serogroups that were tested and showed no cross-reactivity with other bacteria, including Acinetobacter spp., Bordetella spp., Pse...
متن کاملBinax and biotest Legionella urinary antigen kits.
The article of Robert F. Benson et al. entitled “Evaluation of the Binax and Biotest urinary antigen kits for detection of Legionnaires’ disease due to multiple serogroups and species of Legionella” (1) states the following in the abstract: “Eighteen were positive with the Binax kit, and 13 were positive with the Biotest.” This statement is misleading since data in both the article and Table 1 ...
متن کاملInduced Expression of the Legionella pneumophila Gene Encoding a 20-Kilodalton Protein during Intracellular Infection
The eukaryotic protein synthesis inhibitor cycloheximid has been used by many investigators to selectively radiolabel intracellular bacteria. Although cycloheximide has no direct effect on bacterial gene expression, there are concerns that long-term inhibition of the host cell protein synthesis may have secondary effects on bacterial gene expression. Therefore, prior to further identification a...
متن کاملInduced expression of the Legionella pneumophila gene encoding a 20-kilodalton protein during intracellular infection.
The eukaryotic protein synthesis inhibitor cycloheximid has been used by many investigators to selectively radiolabel intracellular bacteria. Although cycloheximide has no direct effect on bacterial gene expression, there are concerns that long-term inhibition of the host cell protein synthesis may have secondary effects on bacterial gene expression. Therefore, prior to further identification a...
متن کاملLaboratory Diagnosis of Pneumonia by Detection of Urinary Antigen Test of Legionella Pnuemophilia
Background and Aims: Legionnaires’ disease is seen in sporadic and epidemic form. The most prevalent cause is Legionella pnuemophilia (L.pneumophila), which produces a severe disease in vulnerable individuals with a high fatality rate. This study was conducted due to the wide and universal distribution of the Legionnaires’ disease, lack of conventional and accessible diagnostic meth...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1987
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.25.11.2080-2084.1987